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technology:dna-editing
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Table of Contents
CRISPR Cas9
Cas 9 enzyme has a sequence locator called the PAM. When the PAM finds a piece of DNA with its sequence, the enzyme unzips the adjacent DNA to see if it matches the guide-RNA. Matching DNA triggers the Cas 9 enzyme to double-strand-break (DSB) the DNA. A DSB is often not a clean break. Some nucleotides can be lost in the process.
The host DNA repair mechanisms come about to repair the DSB, and if a homologous DNA strand is around, it will be used as a template for repair. The homologous DNA can be provided as part of the technological payload, for the purpose of altering the genome.
Zinc Finger
TALENS
Meganucleases
SSRs
technology/dna-editing.1575700964.txt.gz · Last modified: 2019/12/07 06:42 by marcos
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